Dr. Alexander  Limanskii

 

 

Present  Address:                                                                          

Laboratory of New Infectious Diseases                                            

Mechnikov Institute of Microbiology                                                

and Immunology                                                                              

Ukrainian Academy of Medical Sciences

14 Pushkinskaya St.                                                                          

Kharkov, 61057                                                                                              

Ukraine

E - mail: alex.limanskii@mail.ru

 

 

 

 

 

EDUCATION:

B.S.: 1975-1979, Radiophysics and Biophysics, Kharkov State University, Kharkov, Ukraine.

M.S.: 1979-1980, Molecular Biophysics, Kharkov State University, Kharkov, Ukraine.

Diploma title: Spectral and thermodynamic parameters of modified 6-azauracils.

Ph.D.: 1996-1998,  Mechnikov Institute of Microbiology and Immunology, Kharkov, Ukraine.

Thesis title: Detection of viruses and bacteria by polymerase chain reaction. Interaction of microorga- 

                     nisms nucleic acids with antimicrobial and anticancer drugs.

 

ACADEMIC APPOINTMENTS:

2003 - present -  Chief Scientist, Laboratory of New Infectious Diseases, Mechnikov Institute of

                            Microbiology and Immunology, Ukrainian Academy of Medical Sciences,

                            Kharkov, Ukraine.

1996 - 2002 - Senior Scientist, Laboratory of New Infectious Diseases, Mechnikov Institute of

                       Microbiology and Immunology, Ukrainian Academy of Medical Sciences.

1996 - 2001 - Senior Scientist, Laboratory of Slow Infectious Diseases, Institute of Experimen- 

                       tal and Clinical Veterinary Medicine, Ukrainian Academy of Agrarian Sciences.

1988 - 1996 - Researcher, Department of Biophysics, B. Verkin Institute for Low Temperatures

                       Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.

1985 -1988  - Junior researcher, Department of Biophysics, B. Verkin Institute for Low

                       Temperatures Physics & Engineering, Ukrainian Academy of Sciences.

1980 - 1985 - Engineer, Department of Biophysics, B. Verkin Institute for Low Temperatures

                       Physics & Engineering, Ukrainian Academy of Sciences.

 

OTHER PROFESSIONAL POSITIONS:

1997 - 2000 - Chief Scientist, Institute of Cattle Breeding, Kharkov, Ukraine.

1995 - 1996 - Scientist, Regional Medical Genetic Center, Kharkov, Ukraine.

1993 - 1994 - Researcher, Regional Epidemiological Station, Kharkov, Ukraine.

MAJOR RESEARCH INTERESTS:

- Molecular biology of nucleic acids, gene regulation, protein - DNA interaction.

- Mechanisms of DNA - ligand specific interaction, thermodynamics of DNA and DNA drug inte- 

   raction.

- Design of molecular genetic test-systems for gene typing and detection of human and animal viruses and bacteria.

- Sexing of animal embryos by polymerase chain reaction. Preimplantation genetic diagnostics.

- Atomic force microscopy (AFM) of cells, biopolymers, nucleic acids, chromatin; modification of AFM substrates and probes, adhesion and mechanical properties of cells, chemical force microscopy.       

 

Professional experience:

Purification of nucleic acids; equilibrium centrifugation; HPLC and conventional liquid chromatogra-phy; spectrophotometry; thermodynamics of DNA melting; conventional gel electrophoresis; polyme-

rase chain reaction; design of molecular genetic test-system for detection and differentiation of micro-organisms; detection of human and animal viruses and bacteria; imaging cells and biomolecules in air and buffer solutions by atomic force microscopy; force measurements, modification of AFM probes and substrates in amino silane vapour, functionalization of amino modified probes by biopolymers.

 

LIST OF MAIN PUBLICATIONS

 

1. Limanskii A., Sheina G.G., Stepanova T.F., Blagoi Yu.P. Studies of cationic and neutral forms of acridine compounds interaction with nucleic acids. – Studia Biophysica 1984; V. 100: 187-194.

2. Gerasuta N.N., Kratenko I.S., Limanskaya O.Yu., Limanskii A.P., Odrazhij N.K. Detection of he-patitis B virus by polymerase chain reaction. – Ultrasound Prenatal Diagnostics 1994; № 4-5: 85-89 (Russian).

3. Limanskii A.P., Limanskaya O.Yu. Polymerase chain reaction with modified primers. – Ultrasound Prenatal Diagnostics 1995; № 6-7: 71-77 (Russian).

4. Limanskii A.P. Specificity of ligands interaction with nucleic acids. Influence of anthracycline an-tibiotics on molecular melting of DNA with high heterogenecity degree. – Ultrasound Prenatal Diag-nostics 1997; № 8-9: 209-217 (Russian).

5. Limanskii A.P. Specificity of ligands interaction with nucleic acids. Melting of polynucleotides-anthracycline antibiotics complexes. – Ultrasound Prenatal Diagnostics 1997; № 8-9: 218-222 (Russi-an).

6. Limanskii A.P. Detection of cytomegalovirus by polymerase chain reaction. – Ultrasound Prenatal

Diagnostics 1997; № 8-9: 223-230 (Russian).

7. Busol V.A., Limanskaya O.Yu., Limanskii A.P., Tsimbal V.I. Detection of bovine leukaemia virus by polymerase chain reaction. – Veterinary Medicine 1998; 74: 35-44 (Russian).

8. Limanskaya O.Yu., Limanskii A.P., Nehoroshih E.M., Busol V.A., Tsimbal V.I. Detection of bovi-ne leukaemia virus by polymerase chain reaction. – Agrarian Sciences 1999; № 9: 35-39 (Russian).

9. O. Limanskaya, A. Limanskii. Universal test-system for PCR sexing of cattle, sheep, goat and pig embryos. – Journal Biology of Reproduction 1999; V. 60, Suppl. 1: P. 195.

10. Limanskii A.P., Limanska O.Yu., Bezugly N.D. Design of PCR test-system for sexing of farm ani-mal embryos. – Scientific Bulletin of Institute of Cattle Breeding 1999; V.75: 160-165 (Russian).

11. Busol V.A., Limanskaya O.Yu., Limanskii A.P., Tsimbal V.I. PCR test-system for detection of bo-vine leukaemia virus. – Veterinary 1999; № 6: 27-32 (Russian).

12. Limanskaya O.Yu., Limanskii A.P. Test – system for cattle embryos gene typing. – Biological Bulletin 1999, № 2-3: 102-106 (Russian).

13. Limanskaya O.Yu., Limanskii A.P., Bezugly N.D. Preimplantation embryo sexing of farm animals by polymerase chain reaction. – Biotechnology 2000, № 2: 66-72 (Russian).

14. Limanskii A.P., Limanskaya O.Yu., Belozersky V.I., Timchenko E.N., Pohil S. I. Detection of me-

ningococcal infectious agents by polymerase chain reaction. – Clinical Laboratory Diagnostics 2000, № 4: 44-47 (Russian).

15. Krasovsky V.V., Pohil S. I., Limanskii A.P., Limanskaya O.Yu., Timchenko E.N. Detection of Listeria spp. by polymerase chain reaction. – Clinical Laboratory Diagnostics  2000, № 6: 37-41 (Rus-sian).

16. Limanskii A.P. Visualization of cruciform structure in supercoiled DNA by atomic force micro-

scopy. – Biophysics 2000, V. 45, № 6: 1039-1043.

17. Limanskii A.P., Limanskaya O.Yu. The methods of molecular genetics as the basis for the prog-rams  of bovine leukosis eradication. – Russian J. of Biotechnology 2001, № 3: 187-193.

18. Limanskaya O.Yu., Limanskii A.P. Embryo sexing and bovine leukemia virus detection by po-lymerase chain reaction. – Agrarian Sciences 2001, № 3: 34-39 (Russian).

19. Limanskii A.P. Investigation of aminomodified mica as a substrate for nucleic acids atomic force microscopy. – Biopolymers and Cell 2001, V. 17, № 4: 292 – 297 (Russian).

20. Limanskii A.P. Identification of hypervariable and conservative locuses of Listeria sp. genome. –

J. of Microbiology, Epidemiology and Immunobiology 2001, № 5: 62-65 (Russian).

21. Limanskii A.P. Study of ainomodified probes for  atomic force microscopy of biomolecules.– Biopolymers and Cell 2002, V. 18, № 1: 62-70 (Russian).

22. Limanskii A.P., Shapoval V.F., Volyanska N.P., Alsaban A., Kuchma I.Yu. Molecular genetic test-systems for detection and typing of Listeria sp. by polymerase chain reaction. – Mikrobiologichny Zhurnal 2002, V. 64, № 4: 39-49 (Russian).

23. Limanskii A.P., Limanskaya O.Yu. Comparison of primer sets for detection of bovine leukemia virus by polymerase chain reaction. – Bulletin of the Veterinary Institute in Pulawy (Poland) 2002, V. 46, № 1: 27-36.   

24. Limanskaya O.Yu., Limanskii A.P. Analysis of primer sets for PCR-detection of provirus DNA of

bovine leukemia virus and human immunodeficiency virus. – Biopolymers and Cell 2002, V. 18, № 2: 124-130 (Russian).

25. Limanskii A.P. Study of cruciform structure in supercoiled pUC8 plasmid DNA by atomic force microscopy and computer modeling. – Biopolymers and Cell 2002, V. 18, № 5: 401-405 (Russian).

26. Limanskii A.P., Limanskaya O.Yu. Atomic force microscopy: from visualization of DNA and cells to measurement of intermolecular interaction forces. – Biological Bulletin 2001; V. 5, № 1-2:

124-132 (Ukrainian).

27. Lymans’kiy A.P., Lymans’ka O.Yu. Study of microorganisms genome DNA by atomic force mic-

roscopy. – Tsitologia i Genetika 2002, V. 36, № 4: 30-36 (Ukrainian).

28. Limanskii A.P., Volyanskaya N.P., Limanskaya O.Yu., Potejko P.I., Lyapunova A.D., Pasechnik

T.O. Analysis of high conservative insertion IS6110 in Mycobacterium tuberculosis genome. – Medical

Bulletin of Bukovina 2002; V. 6, № 3: 174-177 (Russian).

29. Limansky A., Shlyakhtenko L., Schaus S., Henderson E., Lyubchenko Y. Aminomodified probes for atomic force microscopy. – Probe Microscopy 2002, V. 2, № 3-4: 227-234.

30. Lymans’kyy A.P., Limans’ka O.Yu. Visualization of cyanobacteria by atomic force microscopy. – Tsitologia i Genetika 2003, V. 37, 1: 68-71 (Ukrainian).

31. A. Limanskii. Atomic force microscopy: from DNA and protein imaging to measurement of inter-molecular interactions force. – Advances in Current Biology 2003; V. 123, № 6: 531-542 (in Russian).

32. A. Limanskii, L. Geue, O. Limanskaya, D. Beier Typing of bovine leukemia virus circulated in Ukraine. – Problems of Virology 2004, № 1: 39-44 (Russian).

33. Limanskii A., Limanskaya O. Inverted repeats: computer analysis of microorganisms genome and imaging of cruciform structure in DNA by atomic force microscopy. – In Bioengineered and Bioinspi-

red Systems. Editor A.Rodriguez-Vazquez. Proceedings of SPIE 2003, V. 5119: 337-348.

34. Klimova E., Limanskii A., Rimarenko E., Borisova E. Evaluation of viral persistence for patients

with myasthenia by different methods of investigation. – Ultrasound Prenatal Diagnostics. Suppl. 2000: 246-248.

35. Limanskii A., Limanskaya O., Volyanskii Y. Computer analysis of inverted repeats in Mycobacte-rium tuberculosis genome. – J. of Microbiology, Epidemiology and Immunobiology 2004, № 5: 48-52.

36. Limanskii A., Minukhin V., Limanskaya O., Pavlenko N., Mishina M., Tsyganenko A. Species-specific detection of Proteus vulgaris and Proteus mirabilis by polymerase chain reaction. – J. of Mic-

robiology, Epidemiology and Immunobiology 2005, № 3: 33-39.

37. Lymans’kyi O. Oversupercoiling and compaction of supercoiled DNA. – Tsitologia i Genetika

2005, V. 39, № 2: 64-71 (Ukrainian).

38. Limanskaya O., Rola M., Bicka L., Kuzmak J., Limanskii A. Detection of bovine immunodeficien-cy virus proviral DNA by polymerase chain reaction. – Problems of Virology 2005, V. 50, № 2: 38-43 (Russian).

39. Limanskaya O., Limanskaya L, Limanskii A. S-DNA is oversupercoiled macromolecule with ~2 Ε rise per base pair. – Biopolymers and Cell 2005, V. 21, № 6: 515-524 (Ukrainian).

40. Limanskaya L., Limanskii A. S-DNA, oversupercoiled DNA with 1.94 – 2.19 Ε rise per base pair.

– Molekulyarnaya Biologiya 2006, V. 40, № 1: 122-136.

41. Limanskaya O., Limanskaya L,, Limanskii A. Compaction of supercoiled DNA on the modified

amino mica. – Biopolymers and Cell 2006, V. 22, № 1: 18-28 (Ukrainian).

42. Limanskii A. Visualization of amplicons after polymerase chain reaction. – Biophysics 2006, V.50, 6: 1019-1024.

43. Limanskii A. Amino modified probes with immobilized linkers and proteins for atomic force mic-roscopy. – Tsitologia i Genetika 2006, V. 40, № 1: 84-93 (Ukrainian).

44. Limanskii A. Functionalization of amino modified probes for atomic force microscopy. – Biophy-sics 2006, V. 51, 2: 186-195.

45. Limanskaya L,, Limanskii A. Compaction of single supercoiled DNA molecules adsorbed onto amino mica. – Russian Journal of Bioorganic Chemistry 2006, V. 32, № 5: 494-510.

46. Limanskii A. Visualization of DNA – T7 RNA polymerase complex by atomic force microscopy. – Biopolymers and Cell 2007, V. 23, № 1: 3-13 (Ukrainian).

47. Limanskii A. Visualizing complexes of bacteriophage T7 RNA polymerase with DNA template af-

ter transcription elongation. – Ukrainian Biochemical J. 2007, V. 41, № 1: 65-74 (Ukrainian).

48. Limanskii A. Visualization of RNA transcripts by atomic force microscopy. – Tsitologia i Geneti-

ka 2007, V. 41,  № 2: 12-18 (Ukrainian).

49. Limanskii A. Compaction of single molecules of supercoiled DNA: from duplex to minitoroidal

and spheroidal conformations. – Biophysics 2007, V. 52, № 1:

50. Limanskaya O., Rola M., Bicka L., Kuzmak J., Volyanskii Yu., Pugach N., Novikov S., Limanskii A. Amplification of bovine immunodeficiency virus proviral DNA in experimentally infected animals. – Mikrobiologichny Zhurnal 2007, V. 69, № 1: 52-60 (Ukrainian).

 

PATENTS

1. Ukraine Patent 36260: A.P. Limanskii, O.Yu. Limanskaya Detection method of bovine leukemia virus 25.11.1999; Publ.16.04.2001. Bul. № 3.

2. Ukraine Patent 33324: A.P. Limanskii, O.Yu. Limanskaya, V.F. Babkin, N.D. Bezugly, Yu.L. Vo-lyansky, I.Yu. Kuchma, A.I. Dobroskok, M.V. Babkin. Method of  animal embryos sexing 10.02.1999. Publ.15.02.2001. Bul. № 1.

3. Ukraine Patent 40310: A.P. Limanskii, A. Yu. Volyansky, O.Yu. Limanskaya Detection method of Listeria monocytogenes, L. Ivanovii, L.seeligeri, L.welshimeri  01.12.2000; Publ.16.07.2001.Bul. №6. 4.Ukraine Patent 40371: A. Yu. Volyansky, A.P. Limanskii, O.Yu. Limanskaya Detection method of pathogenic Listeria sp. 22.12.2000; Publ.16.07.2001. Bul. № 6.

5. Ukraine Patent 39715: O.Yu. Limanskaya, A. Yu. Volyansky, A.P. Limanskii Typing method of pa-thogenic and nonpathogenic Listeria sp. 03.01.2001; Publ.15.06.2001. Bul. № 5.

6. Ukraine Patent 66255: Tsyganenko A., Limanskaya O., Limanskii A., Mishina M., Minukhin V. Detection method of Proteus vulgaris 09.09.2003; Publ.15.04. 2004. Bul. № 4.

7. Ukraine Patent 66291: Limanskii A., Limanskaya O., Volyanskii A., Dobroskok A., Novikov S., Manolov V., Volyanskaya N. Detection method of bovine immunodeficiency virus 13.10.2003; Publ.15.04. 2004. Bul. № 4.

8. Ukraine Patent 13571: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Li-manskaya L., Klimov O. Method of mica (as substrate for atomic force microscopy) modification 22.08.2005; Publ.17.04.2006. Bul. № 4.                                                                                                    

9. Ukraine Patent 17942: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Limanskaya L., Klimov O. Method of stretching linear and supercoiled DNA molecules 25.04.2006; Publ. 16.10.2006. Bul. № 10.                                                                                                                              

10. Ukraine Patent 17981: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Kuchma I., Li-manskaya L., Pugach N. Method for compression of linear and supercoiled DNA molecules 03.05. 2006; Publ.16.10.2006. Bul. № 10.

SELECTED ABSTRACTS

1. G.G. Sheina, A.P. Limanskii, V.P. Chernetsky, I.V. Alekseeva, V.F. Pedash. Spectral and quantum

chemical study of physical and chemical properties of 6-azauracil derivatives. - Symposium "Perspec-tives of Bioorganic Chemistry in the New Drugs Design". Riga, 1982; P. 139 (Russian).

2. Limanskii A.P., Pivovarov V.B., Sheina G.G. Spectral study of DNA-aclacinomycin A binding spe-

cificity. - V Conference at Spectroscopy of Biopolymers. Kharkov, 1984; P. 143 (Russian).

3. Limanskii A.P., Pivovarov V.B., Sheina G.G. Interaction of aclacinomycin A with DNA. - VI Sym- posium on Conformational Changes of Biopolymers in Solutions. Tbilisi, 1985; P. 184 (Russian).

4. Limanskii A.P., Plokhotnichenko A.M. Automatic device for study of DNA melting curves fine structure. - XVIII Scientific Technical Conference of Young Scientists of Institute For Low Tempera-ture Physics and Engineering. Kharkov, 1986; P. 141-142 (Russian).

5. Limanskii A.P., Leont'ev V.S., Sheina G.G. Interaction of anthracycline compounds with DNA pAO3 and polynucleotides. – VI Conference on Spectroscopy of Biopolymers. Kharkov, 1988; P. 191-192 (Russian).

6. Pivovarov V.B., Limanskii A.P., Stepan'yan S.G., Sheina G.G. UV- and IR-spectra and structure of

cytosine, isocytosine in organic and water solutions. – VI Conference on Spectroscopy of Biopolymers. Kharkov, 1988; P. 239-240 (Russian).

7. Limanskii A.P. Experimental melting profiles of complexes pAO3 DNA with anthracycline antibio-tics. – XXI Scientific Technical Conference of Young Scientists of Institute for Low Temperature Phy-sics and Engineering. Kharkov, 1990; P. 88-89 (Russian).

8. Limanskii A.P. Interaction of anthracycline antibiotics with heteropolynucleotides. – XXI Confe-rence of Young Scientists of Institute for Low Temperatures Physics and Engineering. Kharkov, 1990; P. 86-87 (Russian).

9. Kamenskii Yu.V., Limanskaya O.Yu., Limanskii A.P. Imaging of oligonucleotides and DNA by scanning tunneling microscopy. – II International Conference on Nanometer Scale Science and Tech-nology. Moscow, Russia, 1993; P. 128.

10. A. Limanskii, O. Limanskaya, Yu. Kamenskii, O. Vovk, L. Vashchenko. Study of fullerene struc-ture by scanning tunneling microscopy. – 185th Meeting of The Electrochemical Society. San Francisco, USA, 1994; P. 215-216.

11. A. Limanskii, O. Limanskaya, Yu. Kamenskii, O. Vovk. Study of fullerene structure by scanning tunneling microscopy. – J. of Electrochemical Society 1994, №.3; P. 1307.

12. Bogatireva R.V., Zhadan I.A., Grechanina Yu.B., Limanskii A.P. Prenatal diagnostics of viral and bacterial embryos affections. – International Scientific Conference devoted to 150-years I.I. Mechni-

kov's birthday. Kharkov, Ukraine, 1995; P. 28 (Russian).

13. N. Gerasuta, I. Kratenko, N. Odrazhij, O. Limanskaya, A. Limanskii. Detection of HIV- and HIV-

associated viruses by polymerase chain reaction. – I National Scientific Practical Conference on HIV Problems. Kiev, Ukraine, 1995; P. 10-11.

14. A.P. Limanskii, O.Y. Limanskaya and E.Y. Grechanina. Polymerase chain reaction with modified

primers. – Proceedings from the First European Symp. on Human Identification, Toulouse, France, 1996. - Promega Corp., 1997; P. 152-153.

15. Limanskii A.P. Diagnostics of meningococcal infection by polymerase chain reaction. – Sanitary

Protection and Prophylaxis of Especially Dangerous Infections in Ukraine. Odessa, Ukraine, 1997; P. 105-106 (Russian).

16. Klimova E.M., Limanskaya O.Yu., Limanskii A.P. Detection of viral hepatitis C, G and B by po-lymerase chain reaction. – Sanitary Protection and Prophylaxis of Especially Dangerous Infections in Ukraine. Odessa, Ukraine, 1997; P. 95 (Russian). 

17. Busol V.A., Limanskaya O.Yu., Limanskii A.P., Tsimbal V.I. Test-system for detection of bovine leukaemia virus by polymerase chain reaction. – International Scientific Practical Conference "Deve-

lopment of Veterinary Science in Ukraine: Achievements and Problems". Kharkov, Ukraine, 1997; P. 108-112 (Russian).

18. Babkin V.F., Limanskaya O.Yu., Limanskii A.P., Nehoroshih E.M., Bezugly N.D. Sexing of far-ming animals embryos. – II International Conference "Modern Molecular Genetic and Biotechnologi-cal Methods in Genetic Selection Research. Odessa, Ukraine, 1998; P. 74 (Russian).

19. Babkin V.F., Busol V.A., Limanskaya O.Yu., Limanskii A.P., Tsimbal V.I. PCR detection of bo-vine leukaemia virus. – II International Conference "Modern Molecular Genetic and Biotechnological Methods in Genetic Selection Research. Odessa, Ukraine, 1998; P. 75-77 (Russian).

20. Limanskii A. Imaging of linear and supercoiled nucleic acids in air and cells in aqueous solution by atomic force microscopy. – Conference for Young Scientists, PhD Students and Students on Mole-cular biology and Genetics. 2001; Kiev, Ukraine, P. 106.

21. Limanska O., Limanskii A. Analysis of primer sets for provirus DNA detection by polymerase chain reaction of bovine leukemia virus and human immunodeficiency virus. – Conference for Young Scientists, PhD Students and Students on Molecular Biology and Genetics. 2001; Kiev, Ukraine, P.150.

22. Limanskii A. Cruciform structure in supercoiled DNA: imaging by atomic force microscopy and

computer modeling. – 8th International Symposium on Molecular Aspects of Chemotherapy. 2001;

Gdansk, Poland, P. 192.  

23. Limanskii A. Atomic force microscopy aminomodified tips and substrates for DNA immobiliza-

tion. – 8th International Symposium on Molecular Aspects of Chemotherapy. 2001; Gdansk, Poland, P.193.  

24. A. Limanskii. Chemical force microscopy of  aminomodified AFM tips and substrates. – Interna-tional Symp. “Scanning Probe Microscopy, Sensors and Nanostructures”. 2001; Tokyo, Japan, P. 85.

25. A. Limanskii. Imaging of cruciform structure in supercoiled DNA by atomic force microscopy. – International Symp. “Scanning Probe Microscopy, Sensors and Nanostructures”. 2001; Tokyo, Japan,  P. 86.

26. Limanskii A.P., Potejko P.I., Lyapunova A.D., Timchenko E.M., Limanskaya O.Yu., Volyanskaya

N.P. Primer set for Mycobacterium tuberculosis detection by polymerase chain reaction. – 8th Ukraini-an Biochemical Symposium. – Ukrainian Biochemical J. 2002; V. 74 (4b), P. 186.

27. A. Limanskii, O. Limanskaya. Inverted repeats in microorganisms: comparison of two isolates of Mycobacterium tuberculosis with complete genome and visualization of cruciform structure in plasmid DNA by atomic force microscopy. – 1st FEMS Congress of European Microbiologists. 2003; Ljublja-na, Slovenia, P. 442.

28. A. Tsyganenko, O. Limanskaya, M. Mishina, A. Limanskii. Molecular genetic analysis of Proteus

mirabilis and Proteus vulgaris genome. – III International Scientific Practical Conference "Science and society social problems: medicine, pharmacy and biotechnology”. 2003; Kharkov, Ukraine, P. 217.  

29. M. Mishina, O. Limanskaya, V. Minukhin, A. Tsyganenko, A. Limanskii. Detection of Proteus mi-rabilis DNA by polymerase chain reaction. – Conference for young scientists, PhD students and stu-dents on Molecular biology and Genetics. 2003; Kiev, Ukraine, P. 22.

30. N. Volyanskaya, A. Limanskii, O. Limanskaya. Development of molecular PCR based assay for detection and differentiation of Mycobacterium tuberculosis complex. – Conference for young scien-tists, PhD students and students on Molecular biology and Genetics. 2003; Kiev, Ukraine, P. 274.

31. A. Limanskii, N. Volyanskaya, O. Limanskaya, Yu. Volyanskii. Primer sets for PCR based detec-tion of coronavirus associated with severe acute respiratory syndrome. – Conference for young scien-tists, PhD students and students on Molecular biology and Genetics. 2003; Kiev, Ukraine, P. 175.

32. Volyanskii Yu., Кleschev N., Sitenko А., Limanskaya O., Limanskii A. Chemical and biological problems of modern counteraction to the international terrorism. – Meeting on the innovative technolo-gies and technical tools for counteraction to the terrorism. 2002; Kharkov, Ukraine, P. 45-47.

33. Limanskaya O, Limanskii A. Imaging of S-DNA – new DNA form with ~2 Ε rise per base pair. – Proc. 15th IUPAB & 5th EBSA International Biophysics Congress. Montpellier, France, 2005; P. 673.

34. Limanskaya O., Limanskii A. Compaction of single supercoiled DNA molecules on the modified aminomica. – Proc. 15th IUPAB & 5th EBSA International Biophysics Congress. 2005;