Scientific Journal "Annalls of Mechnicov's Institute"

 

 

 

 

 

2007 № 2

 

 

Review

Myronenko L. G., Peretyatko E. G.
VANCOMYCIN RESISTANT ENTEROCOCCUS (REVIEW)
In Russian

Papers data about the role of Vancomycin-resistant enterococcus in appearing of nosocomial infection are generalized. The mechanisms of resistance formation to glycopeptidal antibiotics are described. The necessity of microbiological monitoring of vancomycin resistance of enterococcus conducting is grounded.

Experimental research

Pokhil S.I.

PATHOGENIC POTENTIAL OF RAHNELLA AQUATILIS:  THE STUDY OF PATHOGENS FACTORS BEING RESPONSIBLE FOR RAHNELLA ABILITY TO COLONIZE SUSCEPTIBLE TISSUES OF HOST ORGANISM
In Ukrainian

In 24 strains of R. aquatilis, there were revealed pathogens factors which provide these microorganisms with potential to colonize susceptible tissues of host organism: high adhesive activity; ability to produce extracellular metabolites having bacteriocinic and repellent activity to representatives of indigenic microflora of human intestine; antilysozyme activity. R. aquatilis strains showed low level of proteinase and neuraminidase activity and did not produce lecithinase. Thermoregulation effects on intensity action of R. aquatilis pathogens factors.

Vyalykh Zh.E., Sutulova A.A., KilipkoL.V.
 
IDENTIFICATION STRAINS OF YERSINIA SPP. AND TESTING OF RESISTANCE

In Ukrainian
The species identification of freshly isolated strains was performed with the help of biochemical testing (the total number of strains was 68) also the sensitivity of this strains to antibiotics was researched. 95,2% of strains which were researched were resistant to ampicilline, 75,8 % – to chloramphenicol, 59,7 % – to tetracycline, 98,4 – 100,0% of them were sensitive to ciprofloxacine, cephalosporins, aminoglicozids, imipenem.

Yagniuk Yu.A., Mariushchenko A.V., Bacumenko A.V., Volkov A.A., Piatacov A.V.
STUDY OF THE MICROBIAL COMPOSITIONS SPECIALITIES OF THE WOUNDED EXUDATE AFTER OF LAPAROSCOPIC AND LAPAROTOMIC OPERATIONS IN BILIARIC TRACTS

Microbial compositions of the wounded exudate, which from patients, was studied, after laparoscopy and laparotomy surgical interferences in biliaric tracts. It was discovered, that, in comparison with laparotomic operations, after laparoscopic cholecystectomy in the wounded exudate during the first day after operacions the maintenance of E.coli and asporogenic anaerobic bacteries, represented  Bacteroides spp., was increased.

Myronenko L.G., Sauchuk A.I.
DISCOVERY OF GENETIC POLYMORPHISM OF C. DIPHTHERIAE MUSEUM CULTURES IN POLYMERASE CHAIN REACTION WITH ARBITRARY PRIMERS
In Russian

The 12 C. diphtheriae museum cultures were investigated by polymerase chain reaction with arbitrary primer № 45. These cultures were isolated in 1954-1959 in Moscow and in 1991 in Kharkov from the patients with diphtheria and the bacilli carriers. The most typical amplicon profiles the museum cultures were determined. The level of genetic similarity of the museum cultures, which manifests by coincidence of the amplicon  profiles, was determined too. This information will allow supplement the certificates of the museum cultures and creation of the data bank, which contains information about of the pathogen's genome circulating in various regions and different epidemic process intensity.

Yelyseyeva I.V., Babych Ye.M., Sklyar N.I., Volyansky Yu.L., Belozersky V.I., Zhdamarova L.A., Myshukova S.A., Sydorenko T.A., Igumnova N.I., N.I.Yevsyukova V.Yu., Ryzhkova T.A.
SCREENING OF MICROBIAL ORIGIN PREPARATIONS WITH POTENTIAL ADJUVANT PROPERTIES ACCORDING TO THEIR INFLUENCE  UPON PHAGOCYTIC ACTIVITY IN  VITRO
In Ukrainian

Undertaken study is devoted to search of effective microbial origin adjuvants for development immunogenic oral vaccines against diphtheria. It’s investigated influence of filtrates of microbial cultures prepared from strains of Corynebacterium and Lactobacillus spp. upon phagocytic activity of human’s and animal neutrophilic leukocytes in vitro. Filtrates prepared from C. pseudodiphtheriticum  and L. plantarum appeared as the most perspective ones according to investigated indices of phagocytosis.

Volyanskiy A.Y., Nikitchenko Yu.V., Simirenko L.L., Kuchma I.Y.
SUPEROXIDE DISMUTASE ACTIVITY AND ENZYMATICALLY ACTIVE CERULOPLASMIN CONTENT IN BLOOD SERUM OF DIFFERENT AGE RATS IN CONDITIONS OF ADT–ANATOXIN IMMUNIZATION
In Ukrainian

Superoxide dismutase activity and enzymatically active ceruloplasmin content in blood serum of 1-, 3-, 12-, 24- and 32–month old control male Wistar rats, and dynamics of change of these parameters in 3 – and 20 – month old rats during the forming of antitoxic immunity to diphtheria and tetanus anatoxins under immunization by ADT –vaccine were investigated. The activity of enzymes utilizing superoxide radicals characterizes by age peculiarities both before immunization and in dynamics of immune response to vaccination.