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Laboratory
1. Name of research project:
“The development of
molecular-genetic test-systems for
detection of tuberculosis agent and genotyping of mycobacterium”. Project start and accomplishment data:
2001.01-2003.12. Project financing: Academy
of medical Science Ukraine. Head of the project: Volyansky Y.L., Director IMI, Dr. Sc., Prof.; Limansky
A.P., Principal Scientist
of LNLEID, Ph. D. 2. Name of research project: “The
development a express diagnostic and treatment control method (polymerase chain reaction based) for legionella infection”. Project start and accomplishment data:
2002.01-2004.12. Project financing: Academy
of medical Science Ukraine. The collaborating organization:
Ltd “Bioanalytical technolohies” “B.A.T.” (Kharkov,
UA), E-mail: bat@kharkov.com Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNLEID, Ph. D., Principal Scientist; Shagun S.V., Director Ltd “B.A.T.”. 3. Name of research project: “Stady of Bacillus antracis genome organization and desing of molecular
test-systems for detection and typing”. Project start and accomplishment data:
2002.01-2004.12/ Project financing: Academy
of medical Science Ukraine. Head of the project: Volyansky Y.L., Director IMI, Dr. Sc., Prof.; Limansky
A.P., Principal Scientist
of LNLEID, Ph. D. 4. Name of research project: “Effect of laser radiation on biological properties (expression of the pathogenic factors) of the
agents of infectious diseases”. Project start and accomplishment data:
2001.01-2003.12. Project financing: Joint-Stock Company “Center of Laser and
Microwave Technologies” (Kharkov,
UA). The collaborating organization:Research
Institute for Laser Biology and Laser Medicine,
Kharkov State University (RILBLM). E-mail: Laser@VL Kharkov.ua; URL: http://www.medlaser.com.ua. Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNLEID, Ph. D., Principal Scientist; Korobov A.M., Director RILBLM, Ph. D. List of current research projects (2005) opened to collaboration:
1. Name of research project: “Stady of genome organization and desing of molecular
test-systems for detection and typing of human
coronavirus associated with severe acute
respiratory syndrome”. Project start and accomplishment data:
2004.01-2006.12. Project financing: Academy
of medical Science Head of the project: Volyansky Y.L., Director IMI, Dr. Sc., Prof. 2. Name of research project: “Stady of biological properties of Bartonella spp. and development
of laboratory diagnostics methods for cat-scratch disease”. Project start and accomplishment data: 2005.01-2007.12. Project financing: Academy of medical Science Head of the project: Pokhil S.I., Director
Scientific IMI, Head LNLEID, Ph. D., Principal Scientist
Head of Department
•Sergiy
I. Pokhil, Head of
LNLEID, Senior Research Scientist, Ph.D. of medicine
Sc. interests:
Ph.D. Thesis: ”Lysogeny,
bacteriocynogenity, some factors of pathogenicity in Klebsiella:
their applied significance” (1992).
Work address: Mechnicov Institute of Microbiology and Immunology, 14/16, Pushkinskaya Str., Kharkov, 61057, Ukraine
Home address: App.99, Frunse Pr., 26, Kharkov, 61007, Ukraine
E-mail: POKHIL-KHARKIV@mail.ru
•Alexandr P. Limansky, Research scientist,
Ph.D. of biology
Sc. interests: molecular biology, molecular diagnostics, polymerase chain reaction, atomic forse microscopy.
Ph.D. Thesis: ”PCR-detection of viruses and bacteria. Interaction of microorganisms nucleic acids with antimicrobal and anticancer drugs” (1998).
•Olena N. Timchenko, Research scientist,
Ph.D. of medicine
Sc.interests: molecular biology,
DNA restriction patterns,
epidemiological genotyping of bacteria.
Ph.D. Thesis: ”An improvement of molecular-genetic
methods of intraspecific epidemiological typing of clinically significant microorganisms from different taxonomic groups” (2005).
•Valeriy V. Krasovsky, Research scientist,
Ph.D. of medicine
Sc.interests: clinical microbiology,
method of determination of microorganisms viabilities, methods of determination of desinfectants effect.
Ph.D. Thesis: ”Biological properties of bacteria of Listeria species circulating in Ukraine” (1995). •Nila A. Chigirinskaya, research scientist •Yelena A. Repnia, assistent •Oleg V. Beznoschenko, assistent •Tatyana A. Kruglova, assistent •Natalia N. Konoval, laborant •Ludmila G. Sologub, laborant •Olena N. Musatova, laborant Main publications: 1.Pokhil S., Smelanskaya M. Method of sample
preparation for electron microscopy study of fibriae
structures of bacteria// Klinikal and laboratory
diagnostics.1993;(5);22-24 2.Tsiganenko A., Pokhil S. Methods of bacteria
classifications: modern state of the
problem// The medicine problem
bulletin.1995;(9);15-24 3.Tsiganenco A., Pokhil S. Thoughts on modern
classification and nomenclature for bacteria of Salmonella
species// The medicine problem
bulletin.1995;(10);89-100 4.Krasovsky V., Volansky Yu.,
Pokhil S. Characteristics
of biological properties of Listeria strains isolates in 6.Pokhil S., Korniyenko I., Pokhil Sv. Biological
properties and medicinal significance of Rahnella aquatilis//
Bulletin of medicine and biology problems.1996;(4);129-140 7.Pokhil S. Rarely occuring,
atypical and new enteerobacteria//
Microbiol.J.1996;58(4);93-104 8.Tsiganenko A., Pokhil S. To
100-th anniversary of the discovery of Widal's reactions
of agglutination Chistory of serodiagnostics
development// Microbiol.J.1996;58(5);107-109 9.Pokhil S. The biological
properties of adsorbed and nonadsorbed OH-agglutinating sera for the
identification of Rahnella aquatilis bacteria// Microbiol.J.
1997;59(1);70-76 10.Pokhil S., Mackiy N. Criteria
of etiological diagnostics of the acute intestinal
infections caused by Rahnella aquatilis//
Infecious Diseas.1997;(3).10-13 11.Pokhil S. Bacteriological diagnostics of diseases caused
by Rahnella aquatilis// Clin.Lab.Diag.1997; (8); 44-47 12.Timchenko Ye., Volansky
Yu., Pokhil S. Analysis of restriction
patterns of DNA as a method of
modern epidemiological typing of the
pathogenic agents of infection diseases// Bulletin of medicine and
biology problems.1997;(32);78-86 13.Pokhil S., Savin R. Application of divice
"Pulse-Gep1"for conducting gel-electrophoresis in the pulsatins field// Ukrainian Journal of Medical
Equipment and
Technology.1997;(3-4);71-75. 14.Pokhil S., Pokhil Sv.
Intestinal CLV-infection in the 15.Tsiganenko A., Timchenko Ye., Pokhil S. DNA restriction patterns as a modern method
of bacteria classification// Bulletin of medicine and
biology problems.1998;(2);16-26 16.Pokhil S., Krasovsky
V., Timchenko Ye. Assessing the weight of inoculate
applied by Semenikhina's replicator//
Clin.Lab.Diag.1998;(7);34-36 17.Pokhil S. Biological
properties of Rachnella aquatilis strains isolated in different regions// Microbiol.J.1998;60(3);31-36. 18.Pokhil S., Derkach S., Pivnenco T., Pertskhalava T. Identification of species of Enterobacteriace
family producing hydrogen sulphoxide//Microbiol.J.998;60(4);3-14 19.Pokhil S.I. Properties
of Pragia fontium bacteria isolated in the
20.Krasovsky V.V., Vasilyev
N.V., Derkach S.A., Pokhil
S.I. Results of the five-year study of listeriosis
in the 21.Krasovsky V.V., Nadtoka
V.L., Pokhil S.I. Listeria
carriership: a sanitation method// Zh. Microbiol. ( 22.Limansky A.P., Limanskaya O.Yu., Belozersky V.I., Timchenko Ye.N., Pokhil S.I. Development and trials of polymerase chain
reaction for detection of meningococcal infection agent// Clin.
Lab. Diag. ( 24.Pokhil S.I. Evolutionary Phylogenetic Classification of Infections Diseases. - 25.Pokhil S.I. Microbiological
characteristics of Rahnella aquatilis
srtains isolated from patients with diarrea. Vesnic Sum. 27.Volyanskaya N.P., Grechko
V.A. Some tendencies of chemotherapy and chemoprophylactics
of acute inflammatory diseasces in modern clinics//
Vestnik Sum. 28.The method of isolation, identification and longtermin
deposition of pure cultures of Rahnella aquatilis/ Pokhil S.I., Osolodchenko T.P., Volyanska
N.P. et al. In : “Methods of recieving
of pure microorganisms cultures and of their long-time saving in collections:
Works of the stuff of the museum of microorganisms to be pathogenetic
for human”. - 29.The method of isolation, identification and longtermin
deposition of pure cultures of Listeria/ Krasovsky V.V., Pokhil S.I., Osolodchenko T.P. et al. In :
“Methods of recieving of pure microorganisms
cultures and of their long-time saving in collections: Works of the stuff of
the museum of microorganisms to be pathogenetic for
human”. - 30.Culture catalogue: Bacteria. Mycoplasma. Chlamydia. Yeasts-like fungi. Mycelious fungi. Cell Cultures/ Selnikova
O.P., Mavrov I.I., Boschenko
Yu.A., Pokhil S.I., - 31.Timchenko Ye.N.
Genotypic of hospital strains of Staphylococcus aureus
by analysis of the length polymorphism of restrictions of chromosome DNA// Vestnic Khar. State. Univ.
2004;(2); 72-75 32.Limanska O. Yu., Limansky
A. P., Bezugly N. D. Preimplantation
embryo sexing of farm animals by polymerase chain reaction. Biotechnology. (in Russian). 2000; (2); 66-72 33.Limansky A.P. Visualization of cruciform
structure in supercoiled DNA by atomic force
microscopy. – Biofizika. (in
Russian). 2000; 45(6); 1039-1043 34.Limansky A.P. Investigation of aminomodified mica as a substrate for nucleic acids
atomic force microscopy. Biopolymers and Cell.(in
Russian). 2001; 17(4); 292 – 297 35.Limanska O.Yu., Limansky A.P. Test – system for cattle embryos gene
typing. – Biological Bulletin. (in Russian). 1999;
(2-3); 102-106 36.Limanska O.Yu., Limansky A.P. Embryo sexing and bovine leukemia virus
detection by polymerase chain reaction. Agrarian Sciences (in Russian). 2001;
(3); 34-39 37.Limansky A.P., Limanska
O.Yu. Molecular genetic methods as fundament of
bovine leucosis eradication programmes.
Biotechnology. (in Russian). 2001; (3); 40-50 38.Limansky A.P. Identification of hypervariable and conservative locuses
of Listeria sp. genome// Zh.
Microbiol. ( 39.Limansky A. Cruciform structure in supercoiled DNA: imaging by atomic force microscopy and
computer modeling/ 8th International Symposium on Molecular Aspects of
Chemotherapy. September 5-9, 2001; ( 40.Limansky A. Atomic force microscopy aminomodified tips and substrates for DNA immobilization/
8th International Symposium on Molecular Aspects of Chemotherapy. September
5-9, 2001; ( 41.Limansky A. Imaging of linear and supercoiled nucleic acids in air and cells in aqueous
solution by atomic force microscopy/ Molecular biology and genetics.
Conference of young scientists. September 20-22, 2001; ( 42.Limanska O., Limansky
A. Analysis of primer sets for provirus DNA detection by polymerase chain
reaction of bovine leukemia virus and human immunodeficiency virus/ Molecular
biology and genetica. Conference of young
scientists. September 20-22, 2001; ( 43. Tsyganenko A.Ya.,
Pokhil S.I., Timchenko
O.M. Fasks and ways of further improvement genotyping
infections diseases exciters by analysis polymorphism of fragments length
restriction endonuclease of genomic DNA//
Experimental and clinical medicine. 2002; (3); 79-83 44. Maryuschenko A.M., Volyanskaya
N.P., Pokhil S.I. Research of rahnellous
infection exciter’s sensitinty to antibacterial
preparations// Experimental and clinical medicine. 2002;(3); 83-87 45. Pokhil S.I. The study of environments of
the vital activity of Budvicia aquatica
bacteria on territory of the 46. Kozko V.M., Krasovsky
V.V., Volianskaya N.P., Maryuschenko
A.M., Pokhil S.I. The estimation of the spreadind extent of Listeria
pathogenic forms amongst epidemiologically endangered professional groups of
people. Sanitation of Listeria carriers with chlorophyllipt// Annals of the Mechnikov
Institute. 2003;.(4-5); 58-59 47. Varbanets L.D., Ostapchuk
A.N., Pokhil S.I. Characteristics of structure
components of Rahnella aquatilis
lipopolysaccharides// Microb.
Z. ( 48. Smelianskaya M.V., Pokhil
S.I. The place of cytomegalovirus amongst infectious agents isolated from
sperm// Dermatology and Venereology. 2001; 1(11); 70-72 49. Tsyganenko A.Ya.,
Minukhin V.V., Pokhil
S.I. Time to change dogmas. Modern theory and practise
of discovering new pathogen agents of infectiuos
disease. –Kharkiv, 2005. –48 p. 50. Martynov A.V., Timchenco
O.M., Pokhil S.I. Chemically modified specific immunoglobulins for legionelliosis
laboratory diagnostics// Microb. Z. ( 51. Martynov A.V., Timchenco
O.M., Pokhil S.I. Chemical modification for rising lysing activity of bacteriophages//
Microb. Z. ( |
